Chitosan microspheres were prepared by emulsification crosslinking method,and their surface was modified by quaternization.The effects of solvent,reaction time and the ratio of quaternary ammonium reagent C4H9Br to the amount of the microspheres on quaternary ammonium chitosan microspheres were investigated.The results showed that the microspheres were well dispersed in acetonitrile solution,and the quaternized microspheres prepared with the reaction time of 12h and the mass ratio of quaternized reagent to chitosan microspheres of 3∶1 had better effect.The antimicrobial properties of chitosan,chitosan microspheres and quaternized chitosan microspheres were tested.The results showed that the antimicrobial properties of the microspheres were as follows:quaternized chitosan>chitosan microspheres>chitosan,and the antimicrobial rate of quaternized chitosan microspheres was 52.1%.
[1] Mi F L,Shyu S S,Chen C T,et al.Porous chitosan microsphere for controlling the antigen release of Newcastle disease vaccine:preparation of antigen-adsorbed microsphere and in vitro release[J].Biomaterials,1999,20(17):1603-1612.
[2] He S M,Fu W L,Zou M J,et al.Construction and evaluation of SAK-HV protein oral dosage form based on chitosan quaternary ammonium salt-PLGA microsphere[J].Journal of Drug Targeting,2019,27(10):1108-1117.
[3] Wei W,Ma G H,Wang L Y,et al.Hollow quaternized chitosan microspheres increase the therapeutic effect of orally administered insulin[J].Acta Biomaterialia,2010,6(1):205-209.
[4] Hua C,Zhang R H,Bai F,et al.Removal of chromium(Ⅵ) from aqueous solutions using quaternized chitosan microspheres[J].Chinese Journal of Chemical Engineering,2016,25(2):153-158.
[5] 王军,李明春,辛梅华等.N-mPEG-O-季铵化壳聚糖微球的制备及其载药性能[J].化工进展,2013,32(1):140-144.
[6] 任雷雷.季铵化壳聚糖微球的制备及对若干染料的脱色研究[D].南开大学,2007.
[7] Zhang W C,Sun C Y,Zhao Y,et al.One-pot synthesis and characterization of cross-linked quaternized chitosan microspheres as protein adsorbent[J].International Journal of Biological Macromolecules,2011,49(4):688-692.
[8] Wu J,Wei W,Wang L Y,et al.Preparation of uniform-sized pH-sensitive quaternized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method[J].Colloids and Surfaces B:Biointerfaces,2008,63(2):164-175.
[9] Wei W,Wang L Y,Wu J,et al.Quaternized chitosan microspheres as insulin carrier[J].Journal of Biotechnology,2008,136(4):S452-S452.
[10] Wang Y,Dang Q F,Liu C S,et al.Selective adsorption toward Hg(Ⅱ) and inhibitory effect on bacterial Growth Occurring on Thiosemicarbazide-Functionalized chitosan microsphere surface[J].ACS Applied Materials & Interfaces,2018,10(46):40302-40316.
基金资助
广西高校糖资源加工重点实验室开放课题(2016TZYKF07);柳州市科学研究与技术开发计划课题(2017BA30203)
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